Mixed antigen ELISA of Mycoplasma pulmonis and M. arthritidis for diagnosis of murine mycoplasmosis
نویسندگان
چکیده
منابع مشابه
Differential identification of mycoplasma pulmonis and M. arthritidis using PCR-based RFLP.
Mycoplasma pulmonis and Mycoplasma arthritidis were differentially identified using PCR-restriction fragment length polymorphism (RFLP). A genus-specific sequence of mycoplasma was amplified by PCR and the PCR products were digested with the restriction enzyme SmaI. Each PCR product from the four isolates of M. pulmonis was digested with SmaI into two fragments; however, there was no digestion ...
متن کاملClinical, pathological, and molecular investigation of Mycoplasma pulmonis-induced murine respiratory mycoplasmosis in a rat (Rattus norvegicus) colony
Aim Mycoplasma pulmonis (MP) remains potentially important rodent pathogen causing murine respiratory mycoplasmosis (MRM) which may go undiagnosed due to its asymptomatic nature. In the present study, we carried out clinical, pathological, and molecular investigations of MP-induced MRM in a rat colony. Materials and Methods Two female Wistar rats were observed to be diseased in animal facilit...
متن کاملGenome of Mycoplasma arthritidis.
The genomes of several species of mycoplasma have been sequenced. Most of these species rely on the glycolytic pathway for energy production, with the one exception of Ureaplasma, a species that breaks down urea as its principle source of acquiring energy. Several species, including as Mycoplasma arthritidis, are nonglycolytic and can use arginine as their source of energy. Described here are t...
متن کاملCharacterization of the metabolism inhibition antigen of Mycoplasma arthritidis.
The Mycoplasma arthritidis antigen(s) responsible for eliciting metabolism-inhibiting antibodies in rabbits has been partially characterized. Metabolism-inhibiting activity was absorbed from rabbit antisera by intact M. arthritidis cells and membranes but much less so by the soluble cytoplasmic fraction, indicating that the antigen is located on the outer membrane surface. It was stable to peri...
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ژورنال
عنوان ژورنال: Brazilian Journal of Veterinary Medicine
سال: 2018
ISSN: 0100-2430,2527-2179
DOI: 10.29374/2527-2179.bjvm096218